The sprint legend, who had won the 100m, 200m and 4x100m Gold at the same venue during the 2012 Olympics, might not have had a flawless end to his career, but his records over the years will definitely make him a once-in-a-lifetime athlete.
"It was 40 minutes and two medal presentations before our run". Yohan Blake was missing from the team, but Bolt magic proved enough for Jamaica.
"They were holding us too long in the call room", Yohan Blake told the media.
It was the first time Bolt had lost a World Championships 100m final he had started, having been disqualified for a false start in Daegu in 2011.
As Bolt fell to the ground, the leg with the golden shoe giving way, the crowd still went wild because the home team went on to win gold in 37.47 seconds, .05 seconds ahead of the United States.
Jamaica's team doctor later said that the 30-year-old had suffered a hamstring cramp. In fact Usain said to me: 'Yohan, I think this is insane.
"I was on the 2004 Olympic team when we lost to Great Britain then pretty much in the same fashion, with Maurice Greene", said Gatlin at a news conference, before turning to the gold medallists and asking: "What were you doing in 2004?"
"But we've got to make sure we come out and have our sticks right because it'll be another competitive race just like the 100 was, but I'm definitely looking forward to the challenge".
There will be a fair amount of emotion from the stands as Mo Farah also runs in his last track race - the 5,000 metres final.
Gatlin, who beat Bolt to gold in the 100m last Saturday, agreed with the complaints and laid the blame at the television scheduling for the event. "I felt like it happened to me, I was on the corner at the time when it happened, it's devastating", he said.
The 60,000-capacity stadium was primed for one last Bolt show, one last "To the World" pose after a victory, but the injury made it blatantly clear why Bolt is ready to retire.
While Usain Bolt was cheered as he received his bronze medal but Gatlin got the opposite reaction.