The Financial Times reports that an Uber representative stated the appeal had been submitted while "constructive discussions" with TfL continue.
Westminster magistrates court received legal papers from Uber this morning, outlining their appeal against TfL's decision to remove its licence after the department ruled the popular ride-hailing firm wasn't "fit and proper" to operate in London. The first hearing on the issue is expected on December 11.
Uber will continue to operate in London throughout the whole appeal process, which could take up to a year or more.
Uber on Friday filed its appeal against a decision by London authorities not to renew its licence, the U.S. ride-hailing company said.
Today was the last day Uber could appeal TfL's decision to renew its licence and now in the hands of the courts, Londoners could see Uber stick around in the future.
TfL said it took the decision on the grounds of "public safety and security implications".
The authorities have a number of concerns about Uber, including its approach to reporting serious criminal offences and its use of technology that allegedly helps it to evade law enforcement officials. "As our new CEO has said, we are determined to make things right".
Uber has been used by 3.5 million Londoners, developing a loyal fan base by offering convenience and lower prices than traditional "Black Cabs".
Mr Khosrowshahi took over at Uber in August after predecessor Travis Kalanick resigned following a series of scandals.
In an open letter last month, Khosrowshahi apologised for "mistakes" made by Uber.
An online petition to "save" Uber has garnered over 850,000 signatures.