US President Donald Trump is expected to announce on Wednesday that the United States recognises Jerusalem as the capital of Israel and will move its embassy there, breaking with longtime US policy and potentially stirring unrest.
Iran "seriously condemns" the US decision to move its embassy to Jerusalem and its recognition of the city as Israel's capital, read a statement on Wednesday from the Ministry of Foreign Affairs carried by state media.
"And the deprivation of the oppressed Palestinian people from their primary rights in forming an independent Palestinian government with the noble Quds as its capital", it said, using the Arabic name for Jerusalem.
Jerusalem remains at the core of the Israel-Palestine conflict, with Palestinians hoping that East Jerusalem - now occupied by Israel - might eventually serve as the capital of a future Palestinian state.
"We call on Muslim peoples to enter into a big uprising against the plot of transferring the U.S. Embassy to Jerusalem", Rouhani said in a press statement during an event in capital Tehran.
While the region requires stability and security, such illegal moves of the U.S. and Israel could push "the Palestinian state and the whole region" toward instability, Rouhani added.
Iran has long supported a number of Palestinian militant groups opposed to Israel.
Palestinians have demanded that part of Jerusalem be set aside as their capital.
"We believe, given the current conditions, all Islamic nations must become united and take a serious step in countering this wrong, illegal, provocative and gravely unsafe move by the U.S.", Rouhani said.