Critics of the president, including some Republicans, spent Friday blasting the vulgar comments he made behind closed doors.
The statement the African ambassadors issued expresses concern over the Trump administration's apparent increasing denigration of Africa "and people of color".
"There is a serious need for dialogue between the U.S. administration and the African countries", said the AU statement.
She stressed that the U.S. was "much stronger than the sum total of one man".
The UN has also branded as racist, the reported remark branding Haiti, El Salvador and unspecified African nations as "shithole countries".
Donald Trump's alleged remarks have caused outrage across the world with officials in African, European, Latin American countries and the United Nations expressing condemnation and summoning American diplomats in protest.
The outcry over Trump's remarks dominated news coverage on Friday and has complicated efforts to reach a deal on immigration.
Senegal followed suit with foreign minister Sidiki Kaba saying the government "firmly condemned the unacceptable remarks which undermine human dignity, especially of Africa and her diaspora".
Sen. Lindsey GrahamLindsey Olin GrahamGOP and Dems bitterly divided by immigration We are running out of time to protect Dreamers US trade deficit rises on record imports from China MORE (R-S.C.), who Durbin said had confronted Trump over the remark, also released a statement saying he had spoken up to Trump about remarks he disagreed with, though he did not directly confirm Trump's comments.
"First stop would be Osu Castle, Elmina Castle, and the over 40 Forts that detained about 30 million slaves, beaten and shipped out like sardine cans and then I will tell you the history of Africa and why people like you made that a "s***hole" continent".
"The African Union condemns the comments in the strongest terms and demands a retraction of the comment as well as an apology to not only Africans but to all people of African descent around the globe", it said.
"I am an African and very proud of my continent".
Addressing the media ahead of the ANC's January 8 statement in East London, deputy secretary general Jessie Duarte described Trump's comments as "unfortunate".
"Africans and Haitians come from "shithole" countries?"
"Ours is not a shithole country, neither is Haiti or any other country in distress", said Duarte.