Mr Trump's speech came after he met Palestinian President Mahmoud Abbas in Bethlehem in the occupied West Bank earlier in the day and held a private meeting, during which they discussed the now- stalled peace process.
"Mr. President, I appreciate the fact that you went to the Western Wall, and you're the first acting president who has done that". "But I want to thank you especially today for your deep commitment to Israel's security, its wellbeing, and its future. And my meeting with my very good friend Benjamin - I can tell you also that he is reaching for peace".
Trump's speech on Tuesday, delivered at Israel's museum to the Jewish state's culture and history, also stood to reaffirm U.S. commitment to Israel and hold up Judaism's historical ties to the country.
"We want to work with honest intentions to achieve peace", Trump said, adding that both sides have promised to seek a peace deal.
Palestinians across the West Bank and Gaza launched a general strike Monday to protest President Trump's visit and to show solidarity with Palestinian prisoners now on hunger strike in Israeli jails. This has resulted in a lot of uncertainty from both sides into any chances of progress even with Trump's intervention.
Trump was scheduled to visit Yad Vashem, the Israeli Holocaust memorial in Jerusalem, on Tuesday, and will next travel to Rome, where he will meet with Pope Francis on Wednesday.
Watch above, via Fox News.
Sen. Marco Rubio said Tuesday he is not optimistic that a major peace agreement between the Israelis and Palestinians is near, despite recent comments made by President Donald Trump.
"If peace means Israel can no longer retain their nature as a Jewish state or give up control of Jerusalem, if that's peace - that's not peace, that's not going to happen", Rubio said.
Mr Trump, speaking alongside Palestinian President Mahmoud Abbas in Bethlehem, said he hoped the USA could help both parties work out their long-standing differences. "So, we are telling you right now, that Iran will not have nuclear weapons", he said.
President Donald Trump's first visit to Jerusalem as president is being overshadowed by reminders of tumult back home.
"Iran calls for the destruction of Israel".
"The conflict is not between religions", he said.